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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has become observed in many tumors, which leads to chromosome instability, and therefore could contribute to tumorigenesis. This protein was located to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential purpose in advertising cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are already explained. Two additional splice variants ended up noted but in-depth nucleotide sequence data just isn't however readily available.

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Blocking peptides are peptides that bind particularly for the target antibody and block antibody binding. These peptide generally has the epitope acknowledged by the antibody.

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This information outlines even more achievable optimization of mobile and immunoassay parameters to be certain the absolute best final results are...

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This manual outlines further more achievable optimization of cellular and immunoassay parameters to make sure the very best success are...

Antibodies sure to the blocking peptide now not bind into the epitope over the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is particular.

Target Information Cyclin E belongs to the really conserved cyclin spouse and children, whose customers are characterized by a remarkable periodicity in protein abundance through the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead for the temporal coordination of each mitotic celebration. Cyclin E kinds a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S section boundary which is degraded as cells progress as a result of S period.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...

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